Team:Nanjing high school/Basic Parts
Team:rePhoslution/Basic Parts
Favorite basic part: BBa_K3524002 and BBa_K3524000
The part BBa_K3524002 and BBa_K3524000 is bio-sensor, a regulable genetic circuit contains nahR gene and Psal promoter for the detection of salicylic acid. With the appearance of salicylic acid, the protein translated from nahR binds with PsaI promoter and initiates the expression of the enhancer green fluorescent protein (EGFP).
Our team has verified this bio-sensor worked well. The future iGEM teams can exchange the EGFP to other functional proteins to make some significant utiles. For example, our team chooses an essential gene GAPDH, which belongs to Tatumella citrea, which can provide phosphorus for crops to replace the EGFP. As a result, in the presence of salicylic acid, GADPH can be expressed, and T. citrea can survive. Since salicylic acid is a root exudate, this genetic circuit that T. citrea can commensalism with crops.
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